The University of Arizona
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Improved collagen extraction by modified Longin method

T A Brown, D E Nelson, J S Vogel, J R Southon

Abstract


A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein ("collagen") remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.

Keywords


evaluation;temperature;collagen;techniques;proteins;organic compounds;organic materials;bones;methods;C 14;carbon;isotopes;radioactive isotopes;absolute age

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