The University of Arizona

DISTURBANCES OF MESENTERIC LYMPH FLOW AND IN VIVO INTESTINAL LYMPHOCYTE TRAFFICKING DURING EARLY GUT INJURY INDUCED BY ISCHEMIA-REPERFUSION IN RATS

H Yang, Y Jin, M Li, C.H Wang, C.W Tang

Abstract


We sought to define the role of mesentericlymph in the generation of remote organdamage at the early stage of gut ischemiareperfusion(I/R) injury. The measurementof mesenteric lymph flow was carried out bycannulation of mesenteric lymphatics. Thedistribution of in vivo intestinal lymphocytetrafficking was performed by 51Cr labeledlymphocyte and measurement of 51Crlymphocytesdistribution by γ -counter.Endotoxin concentration was assayed usingthe limulus test kit and TNF-α level wasdetected by ELISA. After gut I/R injury, thevolumes of lymph flow in mesentericlymphatics per hour were sharply decreased by72% and the number of intestinal lymphocytesper milliliter was decreased by 61%, which ledto the intestinal lymphocyte output per hoursignificantly decreased by 90% (predominantlyT cells), while the population of 51Crlymphocytesin Peyer’s patches, small intestine(except Peyer’s patches), mesenteric nodes,large intestine, and stomach increased by87%, 191%, 87%, 266%, 262%, respectively.Meanwhile, endotoxin and TNF-α levels inmesenteric lymph were significantly increased.These findings demonstrate the markeddisorders of mesenteric lymph flow and in vivointestinal lymphocytes migration and theaccompanying increase of endotoxin andTNF-α levels in mesenteric lymph in the earlystage of gut I/R injury.

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